The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition. Burnette in 1981 after the eponymous Southern blot for DNA and the consequent coinage of the northern blot in 1977 for RNA.12 Western blotting separates, detects, and identifies one or more proteins in a complex mixture. Load directly on gelsno pre-heating is necessary. The name ‘western’ blot was first coined by Dr. When used during SDS-PAGE, these protein ladder sets provide molecular weight markers ranging from 10250 kDa (unstained ladder) or 10210 kDa (pre-stained ladder). Tested in polyacrylamide gel electrophoresis and Western Blotting. Convenient and ready-to-use CLEARLY Protein Ladder sets are mixtures of 11 highly purified recombinant proteins.
One prominent feature of this protein ladder, is that it remains in the liquid state at the storage temperature therefore, there is not a freeze-thaw cycle involved, making the ladder very stable.Ġ.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25☌), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.įor each application, 5-15µl is needed, depending on the size of the wells. Comparison chart and selection guide for protein gel electrophoresis size standards, ladders, and molecular weight markers (MW markers), including unstained and prestained varieties for in-gel or western blot detection. No need to heat, dilute, or add reducing agent before loading. The ladder is supplied in protein gel loading buffer and is ready-to-use. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. Precision Plus Protein All Blue Standards are a mixture of ten blue-stained recombinant proteins (10250 kD), including three reference bands (25, 50, and 75 kD). Use 5-15µl, depending on the size of the wellsĪpplication: The Bullseye Pre-stained Protein Ladder is suitable for visualizing proteins during electrophoresis without staining and for monitoring transfer onto membrane. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple.2 reference bands - red at ~75kDa and green at ~25kDa.Stoolfix Gut Microbiome Stabilization Kitsīullseye Pre-stained Protein Ladder, 10-180 kDa.Beakers, Graduated Cylinders, and Flasks.Aluminum Foil, Parafilm, and Multiwipes.